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Literature summary extracted from

  • Tan, Z.; Clomburg, J.M.; Gonzalez, R.
    Synthetic pathway for the production of olivetolic acid in Escherichia coli (2018), ACS Synth. Biol., 7, 1886-1896 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.206 synthesis a synthetic pathway for the production of olivetolic acid in Escherichia coli is developed. Through combining OLA synthase and OLA cyclase expression with the required modules of a beta-oxidation reversal for hexanoyl-CoAgeneration, we demonstrate the in vivo synthesis of olivetolic acid from a single carbon source. The integration of additional auxiliary enzymes to increase hexanoyl-CoA and malonyl-CoA, along with evaluation of varying fermentation conditions enabled the synthesis of 80 mg/l olivetolic acid. Olivetolic acid has various pharmacological activities Cannabis sativa
4.4.1.26 synthesis synthesis of olivetolic acid from a single carbon source through combining OLA synthase and OLA cyclase expression with the required modules of a beta-oxidation reversal for hexanoyl-CoA generation. The use of additional auxiliary enzymes to increase hexanoyl-CoA and malonyl-CoA, along with evaluation of varying fermentation conditions, enables the synthesis of 80 mg/L olivetolic acid Cannabis sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.206 expression in Escherichia coli Cannabis sativa
4.4.1.26 expression in Escherichia coli Cannabis sativa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.206 Cannabis sativa B1Q2B6
-
-
4.4.1.26 Cannabis sativa I6WU39
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.206 OLA synthase
-
 Cannabis sativa
2.3.1.206 OLS
-
 Cannabis sativa
4.4.1.26 OAC
-
 Cannabis sativa